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Davids Biotechnologie

Advantages of egg yolk antibodies.

· Easier antibodies against phylogenetic distant to mammallian antigens.

· More antibodies per antigen.

· Additional species for multi-stainings.

· No bleeding of animals.

· Less background in mind with low Fc and low complent binding.

· One hen can produce by laying eggs the amount of antibodies compared to 10 rabbits within one year

· Less energy and resource consumption. Reduced CO2 release compared to rabbit serum antibodies.

· Easy to  breed and keep under animal welfare conditions.

 

Chicken egg yolk antibodies

Immuno precipitation with egg yolk antibodies.

· Egg yolk antibodies do not bind to ProteinA. To make immuno precipitation easy also with egg yolk antibodies, Davids Biotechnologie offers a anti-egg IgG matrix, enabeling the researcher to perform immunoprecipitation  as easy as with ProteinA. You can buy our anti-egg IgG beads: Cat.No.P075.003  0,3 ml.
Cat.No P075.010  1,0 ml

Chicken egg yolk complete package (Cat.No.A001)

Our standard package includes the prepurification according to our DabioPrepI purification.This is an extract which can be used like a serum purified by precipitation with ammonium sulfate.
Preimmune IgY (>90%pure) from one egg yolk, immune IgY from 10 immuno egg yolks (>90% pure), elisa titre detection of antibody fraction. Immunizations will start 1-5 days after arrival of antigens.

Antigen preparations for immunizations.

 

PROTEINS:
1. Amount needed:    0,2 to 0,6 mg for each rabbit is in most cases enough. In some cases it is also enough to immunize with 3 times 5 µg of antigen only. If you have only 20 to 50 µg this in in many cases enough to get good antibodies.
2. Solution: In PBS or 0,9% NaCl is perfect. The pH is best between pH  6 and pH 8. The preparation can contain 1 to 2 M Urea. Please avoid: methanol, guanidine-HCl, toxic substances, organic soluants.
3. If your protein is toxic or only soluble in organic substances we can introduce it into liposomes to overcome problems during the immunization period.
4. Concentration: 0,4 mg/ml to 2 mg/ml is best. Higher concentration are also ok. Lower concentrations are better concentrated. We offer a concentration service for only EUR 34 (Cat.No.A704)  for each antigen.
5. If your protein is within an acrylamid gel matrix this is ok. We offer the preparation of the protein in a gel matrix for only EUR 34 (Cat.A703).

 

PEPTIDES:
1. Amount needed: 1-2 mg for each rabbit or hen  for the immunizations. 3-5mg for an affinity matrix. 1 mg for Elisa tests.
2. Conjugation to KLH or BSA or Ovalbumin or Thyroglobulin or Dextran. If not otherwise indicated we use hemocyanin from moluks (KLH) as this is in most cases the best. Please make use of our peptide synthesis/ peptide conjugation combi pack to save money and time Cat.P101).
3. We can conjugate peptides via amino groups or hydroxyl groups or sulphhydril groups or carboxyl groups or other reactions on demand.

 

CELLS (cell culture cells, bacteria, protozoa, virus particles)

1. Membrane stabilization of membrane structures.
2. Immunization with complete stabilized cells.

3. Optional affinity purification with stabilized and immobilized cells

4. Stabilization KIT is available at Davids Biotechnlogie.

 

 

Getting antibodies from hens is as simple as collecting eggs.

Click here for direct contact:

Info@dabio.de

Egg yolk antibody

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